Posted in Antibodies, Assay Kits, cDNA, Culture Cells, DNA Templates, DNA Testing, Elisa Kits, Equipments, Exosomes, Gels, PCR, Pcr Kits, Peptides, Reagents
Screening of multiclass pesticide residues in honey by SPE-GC/MSD: a pilot study.
The analysis method for monitoring of multiclass pesticide residues (chemical structure variables and chromatographic behavior) in honey has been optimized and in-house validated in this study. Chemical Confirmation of 35 selected pesticides (pesticides and pesticides treated in Hive applied to agricultural practices around Apiaries) have been achieved by extraction of acetonitrile / partition and cleaning by modifying modified EPA solid-phase (SPA) protocol. On the GC / MS pesticide screener Drs.
The extraction procedure applied has provided recovery that can be accepted with related precision (RSD) for chosen pesticides in the range as suggested by Sante on MQL 10 μg KG-1. The potential effect of the matrix for selected analytes is calculated using honey from five different flower sources. Optimized methods are used to determine the level of pesticide residue in honey samples collected randomly from 26 different APIs in Pakistan.
Residue of nine chosen pesticides (diklorvos, mevinphos, ethalflurin, triflurin, lindane, chlorpyrifos-methyl, dieldrin, profenofos, 4,4-DDE) is often detected in a range of 3-48.8 μg kg-1 in 26.9% of The sample analyzed (n = 26) and 15.3% of the sample studied exceeded the maximum residual limit (MRLS). Acaricides, i.e., Coumaphos, Tau-fluvenalate, and Malathion, were not detected in one of the samples of honey analyzed.
Interference exchanges for various hydrocarbons are stunned in part using GC-EI-MSD.
The industrial catalyst process, such as the Fischer-Tropsch process produces various products from Syngas (H2 / CO) such as; Jet fuel, gasoline, diesel, synthetic rubber, monomers for the plastic industry and other oil / candles for special purposes such as cosmetics. Many publications, due to the discovery of the FT process in 1925, has compiled an effort to explain the mechanism.
Many of these publications seek to investigate the FT mechanism with the utilization of certain deuterium experiments through Syngas switching from H2 / CO to D2 / CO. The results of this switching show that hydrogen is involved in limited steps, however; The overall process conversion produces a reverse kinetic isotope effect (IKIE). To confirm the results are not hindered by the physical switch of hydrogen isotopes, further experiments are carried out using the same molar from each of the isotopes competitive (i.e. with molar H2 / D2) / co.
Complications arise from this competitive work because it produces fully exchanged products – all stupied hydrocarbons are partially inseparable by chromatography. Thus, this compound can no longer be separated by chromatography and now requires further separation by the masses. The overall scope for this work is to determine whether a series of paraffin compounds that are partially frustrated, produced by FT, can be analyzed using the EI-MSD without inter-instrumental H / D exchange. The results show that there are no real exchanges on the EI MSD for carbon range from around C6 – C16. Thus, even though the material cannot be separated by chromatographically, they can be separated further and analyzed to determine the entire H / D content for this specific chain length.
Methylation site display (MSD) -aflp, sensitive and affordable method for analysis of CPG methylation profiles.
It has been shown that environmental factors or chemicals can cause developmental diseases. To detect abnormal epigenetic changes in DNA methylation, convenient and cost-effective methods are needed for the study, where some samples are processed simultaneously. We here present the appearance of methylation sites (MSD), unique techniques for DNA library preparation. By combining it with reinforced long fragment length (AFLP) analysis, we developed a new method, MSD-AFLP.

The display library of sites that are monitored only consists of DNA originating from DNA fragments, namely CPG at the end of 5 ‘in the original genomical DNA sample. To test the effectiveness of this method, the level of CPG methylation in the liver, kidney, and mouse hippocampal tissue compared to checking if MSD-AFLP can detect subtle differences in CPG levels that are displaced with specific tissues. As a result, many CPG sites are thought to be specifically different networks detected differently.
The nucleotide sequence adjacent to this metyl-CPG site is identified and we determine the level of methylation with endonuclease methylation restrictions (MSle) –PCR analysis to confirm the accuracy of AFLP analysis. The difference in the level of methylation between networks is almost identical among these methods. With the MSD-AFLP analysis, we detected many CPG showed a statistically significant significant and statistically significant significant difference and a variability rate of less than 10%. In addition, MSD-AFLP analysis can be used to identify CPG methylation sites in other organisms including humans.
Screening with the NMNAT2-MSD platform identifies small molecules that modulate nmnat2 levels in cortical neurons.
Nicotinamide Mononucleotide Adenylyl Transferase 2 (NMNAT2) is a maintenance factor of the main neurons and provides strong neuroprotectors in many preclinical models of neurological disorders. Nmnat2 significantly reduced in Alzheimer’s disease, Huntington, Parkinson. Here we developed a meso-based screening platform (MSD) to calculate endogenous nmnat2 in cortical neurons. High sensitivity and a large dynamic range of the NMNAT2-MSD platform allows us to filter the Lopac Sigma Library consisting of 1280 compounds. This library has a 2.89% hit rate, with 24 positive nmnat2 and 13 negative modulators identified.
Western analysis was carried out to validate and determine the dose of the dependency of the identified modulator. Caffeine, one positive modulator is nmnat2, when it is managed systemicized in the expression of NMNAT2 in RTG4510 mice of tags to normal levels. We confirm in the cell culture model four selected modulators provide special neuroprotectors of nmnat2 to the death of cells induced by Vincristine while four negative modulators are selected to reduce the feasibility of neurons in a way that depends on nmnat2.
ASAH2 Rabbit pAb |
A7985-200ul |
Abclonal |
200 ul |
EUR 459 |
ASAH2 Rabbit pAb |
A7985-20ul |
Abclonal |
20 ul |
EUR 183 |
ASAH2 Rabbit pAb |
A7985-50ul |
Abclonal |
50 ul |
EUR 223 |
ASAH2 Antibody |
24731-100ul |
SAB |
100ul |
EUR 390 |
ASAH2 Antibody |
36240-100ul |
SAB |
100ul |
EUR 252 |
ASAH2 Antibody |
1-CSB-PA076840 |
Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
ASAH2 Antibody |
1-CSB-PA133826 |
Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
ASAH2 Antibody |
1-CSB-PA002170EA01HU |
Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500 |
ASAH2 antibody |
70R-12124 |
Fitzgerald |
100 ug |
EUR 403 |
Description: Rabbit polyclonal ASAH2 antibody |
ASAH2 Antibody |
4743-002mg |
ProSci |
0.02 mg |
EUR 171.82 |
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Description: ASAH2 Antibody: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract. |
ASAH2 Antibody |
4743-01mg |
ProSci |
0.1 mg |
EUR 436.42 |
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Description: ASAH2 Antibody: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract. |
ASAH2 Polyclonal Antibody, HRP Conjugated |
A62107 |
EpiGentek |
100 µg |
EUR 570.55 |
Description: reagents widely cited |
ASAH2 Polyclonal Antibody, FITC Conjugated |
A62108 |
EpiGentek |
100 µg |
EUR 570.55 |
Description: Ask the seller for details |
ASAH2 Polyclonal Antibody, Biotin Conjugated |
A62109 |
EpiGentek |
100 µg |
EUR 570.55 |
Description: The best epigenetics products |
ASAH2 Conjugated Antibody |
C36240 |
SAB |
100ul |
EUR 397 |
ASAH2 siRNA |
20-abx900469 |
Abbexa |
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ASAH2 siRNA |
20-abx908269 |
Abbexa |
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ASAH2 siRNA |
20-abx908270 |
Abbexa |
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ASAH2 Peptide |
4743P |
ProSci |
0.05 mg |
EUR 164.75 |
Description: (CT) ASAH2 peptide |
ASAH2 Antibody, HRP conjugated |
1-CSB-PA002170EB01HU |
Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
ASAH2 Antibody, FITC conjugated |
1-CSB-PA002170EC01HU |
Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
ASAH2 Antibody, Biotin conjugated |
1-CSB-PA002170ED01HU |
Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
ASAH2 cloning plasmid |
CSB-CL865115HU-10ug |
Cusabio |
10ug |
EUR 474 |
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Description: A cloning plasmid for the ASAH2 gene. |
ASAH2 Blocking Peptide |
3828BP-50 |
Biovision |
|
EUR 153 |
ASAH2 Blocking Peptide |
33R-10941 |
Fitzgerald |
50 ug |
EUR 191 |
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of ASAH2 antibody, catalog no. 70R-12124 |
Mouse ASAH2 shRNA Plasmid |
20-abx974501 |
Abbexa |
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Rat ASAH2 shRNA Plasmid |
20-abx987160 |
Abbexa |
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Human ASAH2 shRNA Plasmid |
20-abx961153 |
Abbexa |
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ASAH2 Recombinant Protein (Mouse) |
RP117410 |
ABM |
100 ug |
Ask for price |
ASAH2 Recombinant Protein (Rat) |
RP191108 |
ABM |
100 ug |
Ask for price |
ASAH2 Recombinant Protein (Human) |
RP036760 |
ABM |
100 ug |
Ask for price |
N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
20-abx303129 |
Abbexa |
-
EUR 411.00
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EUR 1845.00
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EUR 599.00
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EUR 182.00
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EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
20-abx007107 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
abx025039-400ul |
Abbexa |
400 ul |
EUR 523 |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
abx025039-80l |
Abbexa |
80 µl |
EUR 286 |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
20-abx214010 |
Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
20-abx214011 |
Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
20-abx177710 |
Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
20-abx173712 |
Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (HRP) |
20-abx303130 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (FITC) |
20-abx303131 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (Biotin) |
20-abx303132 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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ASAH2 ELISA Kit (Human) (OKCD01831) |
OKCD01831 |
Aviva Systems Biology |
96 Wells |
EUR 831 |
Description: Description of target: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.31 ng/mL |
ASAH2 ORF Vector (Human) (pORF) |
ORF012254 |
ABM |
1.0 ug DNA |
EUR 354 |
Asah2 ORF Vector (Rat) (pORF) |
ORF063704 |
ABM |
1.0 ug DNA |
EUR 506 |
Asah2 ORF Vector (Mouse) (pORF) |
ORF039138 |
ABM |
1.0 ug DNA |
EUR 506 |
Asah2 sgRNA CRISPR Lentivector set (Mouse) |
K3499601 |
ABM |
3 x 1.0 ug |
EUR 339 |
ASAH2 sgRNA CRISPR Lentivector set (Human) |
K0130501 |
ABM |
3 x 1.0 ug |
EUR 339 |
Asah2 sgRNA CRISPR Lentivector set (Rat) |
K7087001 |
ABM |
3 x 1.0 ug |
EUR 339 |
Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) Protein |
20-abx654471 |
Abbexa |
-
EUR 578.00
-
EUR 258.00
-
EUR 1720.00
-
EUR 690.00
-
EUR 425.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
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Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
K3499602 |
ABM |
1.0 ug DNA |
EUR 154 |
Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
K3499603 |
ABM |
1.0 ug DNA |
EUR 154 |
Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
K3499604 |
ABM |
1.0 ug DNA |
EUR 154 |
ASAH2 sgRNA CRISPR Lentivector (Human) (Target 1) |
K0130502 |
ABM |
1.0 ug DNA |
EUR 154 |
ASAH2 sgRNA CRISPR Lentivector (Human) (Target 2) |
K0130503 |
ABM |
1.0 ug DNA |
EUR 154 |
ASAH2 sgRNA CRISPR Lentivector (Human) (Target 3) |
K0130504 |
ABM |
1.0 ug DNA |
EUR 154 |
Mouse ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM) |
ELI-34287m |
Lifescience Market |
96 Tests |
EUR 865 |
Human ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM) |
ELI-34330h |
Lifescience Market |
96 Tests |
EUR 824 |
Rat ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM) |
ELI-48960r |
Lifescience Market |
96 Tests |
EUR 886 |
Asah2 sgRNA CRISPR Lentivector (Rat) (Target 1) |
K7087002 |
ABM |
1.0 ug DNA |
EUR 154 |
Asah2 sgRNA CRISPR Lentivector (Rat) (Target 2) |
K7087003 |
ABM |
1.0 ug DNA |
EUR 154 |
Asah2 sgRNA CRISPR Lentivector (Rat) (Target 3) |
K7087004 |
ABM |
1.0 ug DNA |
EUR 154 |
ASAH2 3'UTR Luciferase Stable Cell Line |
TU001220 |
ABM |
1.0 ml |
EUR 1521 |
Asah2 3'UTR GFP Stable Cell Line |
TU250951 |
ABM |
1.0 ml |
Ask for price |
Asah2 3'UTR Luciferase Stable Cell Line |
TU200951 |
ABM |
1.0 ml |
Ask for price |
Asah2 3'UTR GFP Stable Cell Line |
TU152183 |
ABM |
1.0 ml |
Ask for price |
Asah2 3'UTR Luciferase Stable Cell Line |
TU102183 |
ABM |
1.0 ml |
Ask for price |
ASAH2 3'UTR GFP Stable Cell Line |
TU051220 |
ABM |
1.0 ml |
EUR 1521 |
ASAH2 Protein Vector (Human) (pPB-C-His) |
PV049013 |
ABM |
500 ng |
EUR 481 |
ASAH2 Protein Vector (Human) (pPB-N-His) |
PV049014 |
ABM |
500 ng |
EUR 481 |
ASAH2 Protein Vector (Human) (pPM-C-HA) |
PV049015 |
ABM |
500 ng |
EUR 481 |
ASAH2 Protein Vector (Human) (pPM-C-His) |
PV049016 |
ABM |
500 ng |
EUR 329 |
ASAH2 Protein Vector (Human) (pPB-His-MBP) |
PV324138 |
ABM |
500 ng |
EUR 481 |
ASAH2 Protein Vector (Human) (pPB-His-GST) |
PV324139 |
ABM |
500 ng |
EUR 481 |
ASAH2 Protein Vector (Rat) (pPB-C-His) |
PV254814 |
ABM |
500 ng |
EUR 1166 |
ASAH2 Protein Vector (Rat) (pPB-N-His) |
PV254815 |
ABM |
500 ng |
EUR 1166 |
ASAH2 Protein Vector (Rat) (pPM-C-HA) |
PV254816 |
ABM |
500 ng |
EUR 1166 |
ASAH2 Protein Vector (Rat) (pPM-C-His) |
PV254817 |
ABM |
500 ng |
EUR 1166 |
ASAH2 Protein Vector (Mouse) (pPB-C-His) |
PV156550 |
ABM |
500 ng |
EUR 1065 |
ASAH2 Protein Vector (Mouse) (pPB-N-His) |
PV156551 |
ABM |
500 ng |
EUR 1065 |
ASAH2 Protein Vector (Mouse) (pPM-C-HA) |
PV156552 |
ABM |
500 ng |
EUR 1065 |
ASAH2 Protein Vector (Mouse) (pPM-C-His) |
PV156553 |
ABM |
500 ng |
EUR 1065 |
Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
20-abx156834 |
Abbexa |
-
EUR 7378.00
-
EUR 3933.00
-
EUR 911.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
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Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) CLIA Kit |
20-abx494694 |
Abbexa |
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EUR 7973.00
-
EUR 4246.00
-
EUR 981.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
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Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
SEE689Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 4731.5 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids. |
Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
SEE689Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 477.3 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids. |
Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
SEE689Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 639 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids. |
Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
SEE689Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2575.5 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids. |
Many of the positive modulators of the identified NMNAT2 are predicted to increase the concentration of the camp, indicating that neuronal levels of NMNAT2 are strictly regulated by camp signaling. Taken together, our findings indicate that the NMNAT2-MSD platform provides a sensitive phenotypic screen to detect nmnat2 in neurons.